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i scei expression vector pcbascei  (Addgene inc)


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    Structured Review

    Addgene inc i scei expression vector pcbascei
    I Scei Expression Vector Pcbascei, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcbascei+vector/pCBASceI+(Plasmid+%2326477)/pm41687404-83-14-18
    Average 96 stars, based on 293 article reviews
    i scei expression vector pcbascei - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Involvement of the splicing factor SART1 in the BRCA1-dependent homologous recombination repair of DNA double-strand breaks
    Article Snippet: .. Next, the cells were transfected with the pCBASceI vector (expression vector of I-Sce I endonuclease, Addgene #26477) using Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA). .. Cells transfected with pEGFP-N1 vector (Clontech, CA, USA) were concomitantly prepared to monitor transfection efficiency.

    Plasmid Preparation:

    Article Title: Involvement of the splicing factor SART1 in the BRCA1-dependent homologous recombination repair of DNA double-strand breaks
    Article Snippet: .. Next, the cells were transfected with the pCBASceI vector (expression vector of I-Sce I endonuclease, Addgene #26477) using Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA). .. Cells transfected with pEGFP-N1 vector (Clontech, CA, USA) were concomitantly prepared to monitor transfection efficiency.

    Article Title: The FANCM-RMI1/2 complex promotes genomic instability and PARP inhibitor sensitivity in BRCA2-deficicient cells
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). .. All mESCs used in this study were derived from the E14TG2a mouse (strain 129/Ola) embryonic stem cell line (karyotype 40, XY; passage 19, kind gift from Rémi-Xavier Coux and Pablo Navarro). mESCs were grown on 0.1% gelatin-coated flasks or dishes, in 5% CO2 37°C incubators.

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress.
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #2647769) by Infusion cloning. .. Chemical inhibitors used include HU (Sigma, H8627); APH (Abcam, ab142400); CPT (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923). siRNA, morpholinos and plasmid transfection siRNA transfection was performed following Lipofectamine RNAiMAX Transfection Reagent protocol (Invitrogen, 13778075). siRNAs used in this study include siRIF1 (Dharmacon, D-027983-02); siBRCA1 (Dharmacon, L-003461-00); si53BP1 (Thermo Fisher Scientific, S14313); siLuciferase GL2 (Dharmacon, D-001100-01).

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #26477 ) by Infusion cloning. .. Chemical inhibitors used include hydroxyurea (Sigma, H8627); aphidicolin (Abcam, ab142400); camptothecin (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923).

    Expressing:

    Article Title: Involvement of the splicing factor SART1 in the BRCA1-dependent homologous recombination repair of DNA double-strand breaks
    Article Snippet: .. Next, the cells were transfected with the pCBASceI vector (expression vector of I-Sce I endonuclease, Addgene #26477) using Lipofectamine 3000 (Thermo Fisher Scientific, MA, USA). .. Cells transfected with pEGFP-N1 vector (Clontech, CA, USA) were concomitantly prepared to monitor transfection efficiency.

    Article Title: The FANCM-RMI1/2 complex promotes genomic instability and PARP inhibitor sensitivity in BRCA2-deficicient cells
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). .. All mESCs used in this study were derived from the E14TG2a mouse (strain 129/Ola) embryonic stem cell line (karyotype 40, XY; passage 19, kind gift from Rémi-Xavier Coux and Pablo Navarro). mESCs were grown on 0.1% gelatin-coated flasks or dishes, in 5% CO2 37°C incubators.

    Generated:

    Article Title: The FANCM-RMI1/2 complex promotes genomic instability and PARP inhibitor sensitivity in BRCA2-deficicient cells
    Article Snippet: .. The I-SceI expression vector (pFD182), enabling simultaneous expression of I-SceI and mCherry, was generated by inserting an IRES-mCherry cassette immediately after the I-SceI ORF of the pCBASceI vector (gift from Maria Jasin, Addgene plasmid 26477). .. All mESCs used in this study were derived from the E14TG2a mouse (strain 129/Ola) embryonic stem cell line (karyotype 40, XY; passage 19, kind gift from Rémi-Xavier Coux and Pablo Navarro). mESCs were grown on 0.1% gelatin-coated flasks or dishes, in 5% CO2 37°C incubators.

    Construct:

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress.
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #2647769) by Infusion cloning. .. Chemical inhibitors used include HU (Sigma, H8627); APH (Abcam, ab142400); CPT (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923). siRNA, morpholinos and plasmid transfection siRNA transfection was performed following Lipofectamine RNAiMAX Transfection Reagent protocol (Invitrogen, 13778075). siRNAs used in this study include siRIF1 (Dharmacon, D-027983-02); siBRCA1 (Dharmacon, L-003461-00); si53BP1 (Thermo Fisher Scientific, S14313); siLuciferase GL2 (Dharmacon, D-001100-01).

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #26477 ) by Infusion cloning. .. Chemical inhibitors used include hydroxyurea (Sigma, H8627); aphidicolin (Abcam, ab142400); camptothecin (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923).

    Cloning:

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress.
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #2647769) by Infusion cloning. .. Chemical inhibitors used include HU (Sigma, H8627); APH (Abcam, ab142400); CPT (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923). siRNA, morpholinos and plasmid transfection siRNA transfection was performed following Lipofectamine RNAiMAX Transfection Reagent protocol (Invitrogen, 13778075). siRNAs used in this study include siRIF1 (Dharmacon, D-027983-02); siBRCA1 (Dharmacon, L-003461-00); si53BP1 (Thermo Fisher Scientific, S14313); siLuciferase GL2 (Dharmacon, D-001100-01).

    Article Title: The human RIF1-Long isoform interacts with BRCA1 to promote recombinational fork repair under DNA replication stress
    Article Snippet: .. I-Scel-2A-CyOFP1 plasmid was constructed by inserting T2A and CyOFP1 sequences into the pCBASceI vector (addgene #26477 ) by Infusion cloning. .. Chemical inhibitors used include hydroxyurea (Sigma, H8627); aphidicolin (Abcam, ab142400); camptothecin (Merck, 208925); phleomycin (Invivogen, ant-ph-1); Doxycycline (Sigma, D9891); VE-821 (Sigma-Aldrich, SML1415); KU-60019 (Sigma-Aldrich, 531978); PF-477736 (Tocris Bioscience, 4277); NU-7441 (Sigma-Aldrich, SML3923).



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